Practice Questions

92. A plant developmental biologist studies a mutant strain of Arabidopsis thaliana that lacks functional expansin proteins. Phenotypically, this mutation results in severe dwarfism because expansins are the mandatory wall proteins that

A. Catalyze the synthesis of secondary lignin complexes
B. Loosen the hydrogen bonds between cellulose microfibrils to permit turgor-driven wall extension
C. Replicate the circular genome of internal chloroplasts
D. Anchor the desmotubule inside the plasmodesmata pores

Expansins disrupt the non-covalent links between cellulose and hemicellulose, allowing turgor pressure to expand the primary cell wall.

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Jul 3, 2026

91. A patient presenting with muscle weakness and exercise intolerance is diagnosed with a mitochondrial myopathy caused by a point mutation in a mitochondrial tRNA gene. This metabolic disease affects both plant and animal cell lines because mitochondria

A. Are entirely dependent on nuclear genes for all their proteins
B. Possess their own independent, circular DNA and semi-autonomous translation machinery
C. Do not require ATP synthesis to survive
D. Are formed via the direct fusion of lysosomal vesicles

Mitochondria contain their own circular genome and ribosomes; mutations in their tRNA disrupt internal protein translation and ATP synthesis.

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Jul 3, 2026

90. An in vitro biochemical assay demonstrates that the tonoplast of a plant cell contains an abundance of H+-ATPase pumps that actively transport protons from the cytosol into the vacuole. This active transport mechanism establishes a

A. Basic pH inside the vacuole relative to the cytoplasm
B. Highly acidic intra-vacuolar environment essential for activating hydrolytic enzymes
C. Hydrophobic core that excludes all dissolved mineral ions
D. Torsional strain that triggers the immediate destruction of plastids

Pumping protons into the central vacuole lowers its internal pH, creating an acidic environment that powers lysosome-like digestive enzymes.

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Jul 3, 2026

Cellulase breaks down the cellulose microfibrils, and pectinase dissolves the middle lamella, releasing wall-free protoplasts.

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Jul 3, 2026

88. A genetic mutation disrupts the synthesis of clathrin proteins inside an animal cell line. Biochemically, this defect will lead to the selective arrest of

A. Simple passive diffusion of carbon dioxide gases
B. Receptor-mediated endocytosis and coated-vesicle budding
C. The synthesis of ribosomal subunits inside the nucleolus
D. The transport of electrons within the cristae

Clathrin is required to structuralize and coat invaginating pits during endocytosis; its absence blocks the internalization of specific ligands.

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Jul 3, 2026

87. A researcher treats a culture of dividing animal cells with the drug cytochalasin B, which selectively inhibits the polymerization of actin microfilaments. The immediate operational outcome observed in these cells will be the

A. Total arrest of nuclear envelope breakdown during prophase
B. Complete failure of cytokinesis, leaving binucleated cells
C. Inhibition of chromosome movement toward the spindle poles
D. Disruption of the mitochondrial electron transport chain

Inhibiting actin prevents the assembly of the contractile ring, blocking cleavage furrow formation and leaving the cell with two nuclei.

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Jul 3, 2026

86. An experimental plant cell line contains a loss-of-function mutation in the gene encoding the enzyme cellulose synthase. During cell division and growth, this molecular defect will directly manifest as the

A. Inability of the cell to replicate its mitochondrial DNA
B. Structural failure and bursting of newly formed daughter cells during expansion
C. Failure of the spindle apparatus to segregate sister chromatids
D. Sudden transformation of chloroplasts into amyloplasts

Without functional cellulose synthase complexes, the cell cannot build a primary cell wall, causing expanding cells to burst under turgor pressure.

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Jul 3, 2026

85. The biochemical nature of the enzyme catalase, which resides inside the peroxisomes of both plant and animal cells, is a

A. Structural lipid matrix
B. Conjugated globular protein (enzyme)
C. Unbranched homopolysaccharide
D. Single-stranded ribozyme

Catalase is a tetrameric protein enzyme containing four heme groups, optimized to break down hydrogen peroxide into water and oxygen.

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Jul 3, 2026

84. The primary biochemical function of the carbohydrate component that forms the outer glycocalyx coating of animal cells is

A. Serving as a template for DNA replication
B. Cell-to-cell recognition and immune system identification
C. Driving the electron transport chain
D. Storing high-density triacylglycerol reserves

The branched sugar chains of the glycocalyx act as molecular tags, enabling the immune system to recognize self cells and coordinate interactions.

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Jul 3, 2026

Integrins are transmembrane receptor proteins that link the intracellular cytoskeleton to the extracellular matrix, facilitating cell signaling.

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Jul 3, 2026
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